Konke Odinga Ukukwazi Nge-Sparger ku-Fermenter

Iyini i-Sparger ku-Fermenter?

Ngamafuphi, u-AI-Sparger ku-Fermenteriyidivayisi Esetshenziselwa Ukwethula Umoya noma Amanye Amagesi Emkhunjini Wokuvutshelwa.

Ngokuvamile ipayipi elinembobo elitholakala ngaphansi komkhumbi noma eduze kwe-impeller futhi ivumela igesi ukuthi ikhishwe oketshezini ngezimbobo ezincane noma amajethi.

Injongoi-sparger iwukunikeza umoya-mpilo wezinqubo zokuvutshelwa kwe-aerobic noma ukuxuba isikhutha (co2) ngezinqubo zokuvutshelwa kwe-anaerobic. i-sparger isiza ukugcina izinga eliphezulu le-oxygen encibilikile endaweni yesiko, ebalulekile ekukhuleni nasekusebenzeni kwe-metabolism yamagciwane, njengemvubelo noma amagciwane.

Phakathi nenqubo yokuvutshelwa, i-sparger ingase isetshenziselwe ukulawula i-PH, izinga lokushisa nokuxuba okuqukethwe komkhumbi. ijwayele ukusetshenziswa ekucutshungulweni kwezinto eziphilayo esikalini sezimboni, lapho umthamo omkhulu wama-microorganisms ukhuliswa ngasikhathi sinye, ukuze kuqinisekiswe ukuvutshelwa okungaguquki nokusebenza kahle.

Emkhakheni wokuvutshelwa, ama-spargers adlala indima ebalulekile ekudaleni indawo efanele yokukhula kwamagciwane. Ngamagama alula, i-sparger iyithuluzi elisetshenziselwa ukwethula igesi (ngokuvamile i-oxygen noma i-carbon dioxide) endaweni enguketshezi. Ngesikhathi sokuvutshelwa, ama-spager avame ukusetshenziselwa ukulawula ukugcwala komoyampilo oncibilikisiwe, isakhi esibalulekile sokukhula kwamagciwane. Kulokhu okuthunyelwe kwebhulogi, sizoxoxa ngokuningiliziwe ukuthi siyini isisakazeli, sisebenza kanjani, nokubaluleka kwaso enqubweni yokuvutshelwa.

Yini umjovo?

I-sparger iyithuluzi elisetshenziselwa ukwethula igesi (imvamisa i-oksijini noma i-carbon dioxide) endaweni enguketshezi. Abasabalalisi ngokuvamile bahlanganisa i-gas inlet kanye nezinto ezimbotshana zokuhlakaza igesi oketshezini. Izinto ezinama-porous zingenziwa ngezinto ezihlukahlukene ezihlanganisa izinsimbi ze-sintered, i-ceramics noma ama-polymers.

Ama-Sparger avame ukusetshenziselwa ukulawula ukugcwala komoyampilo oncibilikisiwe emithonjeni yoketshezi ngesikhathi sokuvutshelwa. Lokhu kungenxa yokuthi i-oxygen iyisakhi esibalulekile sokukhula kwe-microbial futhi ukutholakala kwayo kuthinta kakhulu isivinini nokusebenza kahle kokuvutshelwa. Ngaphezu kwalokho, othisha bangasetshenziswa futhi ukulawula i-pH yendawo yokuvutshelwa ngokwethula i-carbon dioxide noma amanye amagesi ane-acidic.

Uhlobo lomsabalalisi:

Kunezinhlobo eziningana zezisakazi ezivame ukusetshenziswa embonini yokuvutshelwa, ngayinye inezinzuzo zayo kanye nokubi. Izinhlobo ezivame kakhulu zokusabalalisa yilezi:

1. Abasabalalisi abanezimbotshana:

Laba basabalalisi benziwe ngezinto ezinama-porous ezifana nensimbi e-sintered, i-ceramics noma ama-polymers. Zivame ukusetshenziswa embonini ngenxa yokusebenza kahle kwazo okuphezulu nokusebenziseka kalula.

2. Izijovo zebhamuza:

Lawa ma-injection angenisa igesi endaweni yokuvubela ngendlela yamabhamuza. Awasebenzi kahle kunama-spargers anezimbotshana, kodwa avame ukusetshenziselwa ukuvutshelwa kwezinga elincane.

3. I-Membrane spagers:

Lezi spargers zisebenzisa ulwelwesi lwegesi olukwazi ukungena ngaphakathi ukuze lungenise igesi endaweni yokuvubela. Ziyasebenza, kodwa kunzima ukuzihlanza nokuzigcina.

Ukubaluleka kwama-spager kuma-fermenters:

Ama-Sparger adlala indima ebalulekile enqubweni yokuvutshelwa ngokulawula ukugcwala komoyampilo oncibilikisiwe kanye ne-pH endaweni yokuvutshelwa. Ukugcwala komoyampilo oncibilikisiwe kuyipharamitha ebalulekile ekukhuleni kwamagciwane njengoba kuthinta izinga nokusebenza kahle kokuvutshelwa. Ngokulawula ukugcwala komoyampilo oncibilikisiwe, ama-spager angasiza ekuqinisekiseni ukuthi inqubo yokuvutshelwa ilungiselelwe isivuno esiphezulu nekhwalithi.

Ngaphezu kokulawula ukugcwala komoyampilo oncibilikisiwe, ama-nebulizers angasetshenziswa futhi ukulawula i-pH yendawo yokuvutshelwa. Lokhu kubalulekile ngoba i-pH ithinta kakhulu izinga nokusebenza kahle kokukhula kwamagciwane. Ngokwethula amagesi e-asidi afana ne-carbon dioxide, ama-spager angasiza ukugcina ibanga elilungile le-pH lokukhula kwe-microbial.

Izinto okufanele uzicabangele lapho ukhetha isipredishithi:

Lapho kukhethwa i-sparger ye-fermenter, izici eziningana zidinga ukucatshangelwa, okuhlanganisa uhlobo lwezilwanyana ezincane ezikhuliswayo, ubukhulu be-fermenter, nezimo ezifiswayo zokuvutshelwa. Isibonelo, ama-spargers anezimbotshana ngokuvamile afaneleka kangcono ukuvutshelwa ngezinga elikhulu ngenxa yokusebenza kahle kwawo okuphezulu, kuyilapho amabhamuza omoya angakufanelekela kangcono ukuvutshelwa kwezinga elincane. Ukukhethwa kwe-sparger nakho kungancika ezidingweni ezithile zenqubo yokuvutshelwa, njengokugxiliswa komoyampilo odingekayo oncibilikisiwe noma ububanzi be-pH obuphelele.

Ukunakekelwa Nokuhlanza Isisabalale:

Ukugcinwa kahle kwe-sparger nokuhlanza kubalulekile ukuze kuqinisekiswe ukusebenza kahle nokuvimbela ukungcola. Ama-Sparger kufanele ahlanzwe ngezikhathi ezithile ukuze asuse noma yikuphi ukunqwabelana kokukhula kwamagciwane noma okunye ukungcola okungase kuthinte ukusebenza kahle kwawo. Inqubo yokuhlanza ingase ihluke kuye ngohlobo lwe-sparger, kodwa ngokuvamile ihilela ukucwilisa i-sparger esixazululweni sokuhlanza, okulandelwa ukugeza okugcwele ngamanzi. Ngaphezu kokuhlanza njalo, i-sparger kufanele ihlolwe njalo ukuze iwonakele noma iguge futhi ishintshwe njengoba kudingeka.

Izinhlobo zama-spager asetshenziswa ku-fermenters

1. I-Porous Distributor:

Ama-spargers anezimbotshana enziwe ngezinto ezahlukahlukene ezihlanganisa izinsimbi ezisikiwe, i-ceramics nama-polymers. Lezi spargers zinendawo ephezulu kanye nenani elikhulu lezimbotshana ezincane, ezivumela igesi ukuthi isatshalaliswe ngokulinganayo kuyo yonke indawo yokuvutshelwa. Ukusebenza kahle okuphezulu kwama-spargers anezimbotshana kuwenza abe yinketho ethandwayo yokuvutshelwa kwesilinganiso esikhulu, lapho amazinga aphezulu okudluliswa kwegesi ebaluleke kakhulu ekukhuleni kwe-microbial. Ama-spargers anezimbotshana anenzuzo yokuba lula ukuwahlanza nokuyinakekela, njengoba ngokuvamile aklanyelwe ukuhlakazwa futhi ahlanzwe phakathi kwamaqoqo.

2. Isidubuli sebhamuza:

Izijovo zamabhamuza, ezaziwa nangokuthi ama-diffuser, zethula igesi endaweni yokuvubela ngendlela yamabhamuza. Lawa ma-spager ngokuvamile enziwa ngezinto ezimbotshana noma ezinezikhala ezinhle ezidala amabhamuza amancane njengoba igesi idlula. Ama-injection amabhamuza ngokuvamile asetshenziselwa ukuvutshelwa kwezinga elincane lapho amazinga aphezulu okudluliswa kwegesi engabalulekile kangako. Ama-bubble sparger anenzuzo yokuthi alula futhi angabizi ukuwasebenzisa, kodwa angase angasebenzi kahle kunama-spargers anezimbotshana futhi adale isiyaluyalu endaweni yokuvutshelwa.

3. Umsabalalisi we-Membrane:

Ama-Membrane spagers asebenzisa ulwelwesi lwegesi olukwazi ukungena ngaphakathi ukwethula igesi endaweni yokuvubela. Lezi spargers zinendawo ephezulu kanye nenani elikhulu lezimbotshana ezincane, ezivumela igesi ukuthi isatshalaliswe ngokulinganayo kuyo yonke indawo yokuvutshelwa. Ama-Membrane spagers asebenza kahle futhi angadlulisela igesi ngesivinini esikhulu, okuwenza abe yinketho edumile yokuvutshelwa kwezinga elikhulu. Ama-membrane spagers, nokho, angaba nzima kakhulu ukuwahlanza nokuyinakekela ngoba ulwelwesi luvame ukuntekenteke futhi lulimale kalula.

4. Ama-aera angaphezulu:

Ama-aera angaphezulu, awaziwa nangokuthi ama-agitator, adala ukugeleza okunesiyaluyalu endaweni yokuvutshelwa, okuvumela igesi ukuthi imunce oketshezini. Laba basabalalisi ngokuvamile basetshenziswa ekuvubeleni kwezinga elincane lapho amazinga aphezulu okudluliswa kwegesi engabalulekile kangako. Ama-aera angaphezulu anenzuzo yokuba lula futhi angabizi ukuwasebenzisa, kodwa angase angasebenzi kahle kunezinye izinhlobo zama-spager futhi adale isiyaluyalu endaweni yokuvutshelwa.

5. Isirinji:

Ama-Eductors asebenzisa amajethi egesi anomfutho ophezulu ukudala isiphithiphithi endaweni yokuvutshelwa, okubangela ukuthi igesi imuncwe oketshezini. Lawa ma-spreaders ngokuvamile asetshenziswa ekuvubeleni kwezikali ezincane futhi anenzuzo yokuthi kulula ukuwasebenzisa futhi angabizi. Kodwa-ke, ama-injection angase angasebenzi kahle kunezinye izinhlobo zama-spager futhi adale isiyaluyalu endaweni yokuvutshelwa.

Khetha isipredishithi esifanele:

Lapho kukhethwa i-sparger ye-fermenter, izici eziningana okudingeka zicatshangelwe, njengohlobo lwezilwanyana ezincane ezikhuliswayo, ubukhulu be-fermenter, nezimo zokuvutshelwa ezifunwayo. Ukukhethwa kwe-sparger nakho kungancika ezidingweni ezithile zenqubo yokuvutshelwa, njengokugxiliswa komoyampilo odingekayo oncibilikisiwe noma ububanzi be-pH obuphelele. Ukugcinwa kahle kwe-sparger nokuhlanza kubalulekile ukuze kuqinisekiswe ukusebenza kahle nokuvimbela ukungcola. Ngokuqonda izinhlobo ezahlukene zezisakazi ezitholakalayo kanye nobuhle nobubi bazo, ochwepheshe bokuvutshelwa bangenza izinqumo ezinolwazi mayelana nendlela yokuthuthukisa inqubo yokuvutshelwa futhi bathole imiphumela efiselekayo.

Umsebenzi oyinhloko we-sparger ku-fermenter

1. Ukukhipha umoya-mpilo:

Omunye wemisebenzi eyinhloko ye-sparger emvubelo ukunikeza umoya-mpilo kumagciwane akhuliswayo. Lokhu kubaluleke kakhulu kuma-microorganisms e-aerobic adinga umoya-mpilo ukuze akhule kanye ne-metabolism. I-sparger iletha umoya-mpilo endaweni yokuvubela ngendlela yamabhamuza omoya noma ukugeleza komoya, okwenza ama-microbe akwazi ukumunca umoya-mpilo futhi awusebenzisele ukukhiqiza amandla.

2. Imiksi:

Ngaphezu kokunikeza umoya-mpilo, i-sparger nayo idlala indima ebalulekile ekuhlanganiseni indawo yokuvubela. Uma igesi yethulwa phakathi nendawo, idala isiphithiphithi, esiza ukusabalalisa izakhamzimba nezinye izithako ezibalulekile ngokulinganayo kulo lonke uketshezi. Ukuxubana ngendlela efanele kubalulekile ukuze kuqinisekiswe ukukhula okuhle kwe-microbial njengoba kuvumela ama-microorganisms ukuthi athole imisoco ayidingayo futhi asuse imikhiqizo engcolile.

3. Ukulawula i-PH:

Ama-Sparger angasetshenziswa futhi ukulawula i-pH yendawo yokuvubela ngokwethula i-carbon dioxide noma amanye amagesi ane-asidi oketshezini. Lokhu kusiza ukugcina i-pH ingaphakathi kwebanga elifunekayo futhi kuvimbele ukukhula kwamagciwane ayingozi achuma ezindaweni ezine-asidi.

4. Ukulawula izinga lokushisa:

Kwezinye izimo, ama-spargers angasetshenziswa ukulawula izinga lokushisa le-fermentation medium. Ngokwethula ukugeleza komoya opholile, i-sparger ingasiza ekunciphiseni izinga lokushisa kwabezindaba futhi ivimbele ukushisa ngokweqile. Ngokuphambene, umoya oshisayo ungasetshenziswa ukwandisa izinga lokushisa lendawo ephakathi futhi ukhuthaze ukukhula kwe-microbial.

5. Ukulawula Igwebu:

Okokugcina, izifutho zingasetshenziswa futhi ukulawula ukwakheka kwegwebu ngesikhathi sokuvutshelwa. Igwebu lingaba yinkinga ekuvubeleni okukhulu njengoba liphazamisa inqubo yokuxuba futhi linciphisa ukusebenza kahle komsabalalisi. Ngokwethula ukugeleza komoya endaweni yokuvubela, ama-spager angasiza ukuphula igwebu futhi avimbele ukunqwabelana kwegwebu.

Ekuphetheni:

Kafushane, ama-spargers adlala indima ebalulekile enqubweni yokuvutshelwa ngokunikeza umoya-mpilo, ukuxuba imidiya yokuvutshelwa, ukulawula i-pH nezinga lokushisa, nokuvimbela ukwakheka kwegwebu. Ukukhetha uhlobo olufanele lwe-sparger nokuyigcina nokuyihlanza ngendlela efanele kubalulekile ekwandiseni inqubo yokuvutshelwa kanye nokuzuza imiphumela oyifunayo. Ngokuqonda umsebenzi womsabalalisi ku-fermenter, ochwepheshe bokuvutshelwa bangenza izinqumo ezinolwazi mayelana nendlela yokuthuthukisa inqubo yokuvutshelwa futhi bazuze imiphumela oyifunayo.

Isicelo of distributor in fermenter

1. Ukukhiqizwa kukabhiya newayini:

Esinye sezicelo ezivame kakhulu zokusabalalisa ama-fermenters ukukhiqizwa kobhiya newayini. Kulezi zimboni, ama-spager avame ukusetshenziselwa ukunikeza umoya-mpilo imvubelo ngesikhathi sokuvutshelwa, okusiza ukukhuthaza ukukhula kwemvubelo okunempilo nokuvutshelwa okuphumelelayo. Ama-Sparger angasetshenziswa futhi ukusiza ukulawula izinga lokushisa kanye ne-pH yendawo yokuvubela, okubalulekile ekuzuzeni ukunambitheka okufiswayo kanye nezinga lotshwala emkhiqizweni wokugcina.

2. I-Bioremediation:

Ama-Spargers nawo asetshenziswa emkhakheni we-bioremediation, ohilela ukusetshenziswa kwama-microorganisms ukudiliza izinto ezingcolisayo nezinye izinto ezingcolisa imvelo. Kulolu hlelo lokusebenza, ama-spager asetshenziselwa ukwethula umoya-mpilo endaweni engcolile, okusiza ukukhuthaza ukukhula kwama-microorganisms anesibopho sokudiliza ukungcola. Le nqubo iphumelela kakhulu ekuhlanzeni inhlabathi namanzi angaphansi komhlaba angcoliswe imikhiqizo kaphethiloli, izincibilikisi namanye amakhemikhali ezimboni.

3. Ukukhiqizwa kwemithi:

Abasabalalisi baphinde basetshenziswe kabanzi embonini yezemithi ukukhiqizwa kwemithi yokugoma, ama-antibiotic kanye neminye imithi. Kulezi zinhlelo zokusebenza, ama-spager asetshenziselwa ukwethula umoya-mpilo kumasiko amagciwane anesibopho sokukhiqiza umkhiqizo ofunekayo. Ama-Sparger angasiza futhi ukuxuba indawo yokuvubela futhi alawule izinga lokushisa kanye ne-pH yesiko, okubalulekile ekugcineni impilo nokukhiqiza kwamasiko amancane.

4. Ukuvutshelwa kwezimboni:

Ama-Sparger nawo avame ukusetshenziswa ekuvubeleni kwezimboni ezinkulu ukukhiqiza ama-enzyme, ama-amino acid, neminye imikhiqizo yebhayoloji. Kulezi zinhlelo zokusebenza, ama-spager asetshenziselwa ukunikeza umoya-mpilo kusiko le-microbial futhi usize ukugcina izinga lokushisa ne-pH edingwa isiko. Ukuzalanisa ngendlela efanele kubalulekile ekuzuzeni isivuno esikhulu kanye nokugcina impilo nokukhiqiza kwamasiko e-microbial.

5. Ucwaningo Nentuthuko:

Okokugcina, abasabalalisi bavame ukusetshenziswa kuzilungiselelo zocwaningo nentuthuko ukufunda ukukhula nokuziphatha kwama-microorganisms endaweni elawulwa ilabhorethri. Ama-Spargers angasetshenziswa ukwethula izinhlobonhlobo zamagesi emithonjeni yokuvutshelwa, okuvumela abacwaningi ukuba bafunde imiphumela yezimo zemvelo ezihlukene ekukhuleni kwamagciwane kanye nemetabolism.

Ekuphetheni

Kafushane, ama-spargers ayingxenye ebalulekile yezinqubo eziningi zokuvutshelwa, ukuhlinzeka nge-oxygen, ukuxuba imidiya yokuvutshelwa, nokulawula izinga lokushisa ne-pH yesiko. Ukusetshenziswa kwama-spreaders kubalulekile ekuzuzeni isivuno esiphezulu nokugcina impilo nokukhiqiza kwamasiko amancane. Ngokuqonda ukusetshenziswa okuhlukahlukene kanye nokusetshenziswa kwezisakazi ekuvubeleni, ochwepheshe bokuvutshelwa bangenza izinqumo ezinolwazi mayelana nendlela yokuthuthukisa inqubo yokuvutshelwa nokuzuza imiphumela efiselekayo.

Isiphi isifutho esidumile se-Fermenter?

i-nozzle sprayer ku-fermenter kanyeAbasabalalisi be-Orifice kuma-fermenters

Ama-nozzles kanye nama-orifice distributors yizinhlobo ezimbili ezidumile zabasabalalisi ezisetshenziswa kuma-fermenters. Nalu ulwazi olwengeziwe ngakunye:

1. Abasabalalisi be-Nozzle kuma-fermenters:

I-nozzle sparger i-sparger esebenzisa uchungechunge lwamabhomu ukwethula amabhamuza omoya endaweni yokuvubela. Imibhobho ivamise ukuhlelwa ngephethini yegridi ezansi kumvubelo lapho igesi yethulwa khona ngenani elilawulwayo. Ama-Nozzle spagers adumile ekuvutshelweni kwesilinganiso sezimboni ngenxa yokuthi ahlinzeka ngokuxutshwa okusebenzayo kanye nomoya-mpilo wendawo yokuvutshelwa. Futhi kulula ukuwahlanza nokuyinakekela.

2. Abasabalalisi be-Orifice kuma-fermenters:

I-orifice sparger iyi-sparger esebenzisa uchungechunge lwezimbobo ezincane noma ama-orifices ukwethula amabhamuza omoya endaweni yokuvubela. Ama-orifices ngokuvamile ahlelwa ngephethini yegridi ngaphansi kwe-fermenter lapho igesi yethulwa khona ngenani elilawulwayo. Ama-Orifice spagers adumile ezindaweni ze-R&D ngenxa yokuthi avumela ukulawula okunembile kwamazinga okwethulwa kwegesi futhi angasetshenziswa ukutadisha imiphumela yezinhlobo ezahlukene zegesi ekukhuleni kwe-microbial kanye ne-metabolism.

Kokubili ama-nozzles nama-orifice spagers asebenza ngempumelelo ekunikezeni umoya-mpilo kusikompilo lwe-microbial kanye nokuhlanganisa indawo yokuvubela. Ukukhethwa kwe-sparger kuncike ezidingweni ezithile zenqubo yokuvutshelwa, okuhlanganisa ubukhulu be-fermenter, uhlobo lwezilwanyana ezincane ezikhuliswayo, nomphumela ofisayo wokuvutshelwa. Ekugcineni, umgomo uwukukhetha i-sparger ehlinzeka ngokudluliswa kwegesi ephumelelayo futhi ikhuthaze ukukhula kwe-microbial enempilo ngenkathi inciphisa ukwakheka kwegwebu kanye neminye imiphumela emibi engathandeki.

Ekuphetheni:

Kafushane, i-sparger iyingxenye ebalulekile yenqubo yokuvutshelwa futhi idlala indima ebalulekile ekulawuleni ukugcwala komoyampilo oncibilikisiwe kanye ne-pH endaweni yokuvutshelwa. Ukukhetha uhlobo olulungile lwesipredishithi nokusinakekela nokuyihlanza ngendlela efanele kungasiza ekuqinisekiseni inqubo yokuvutshelwa elungiselelwe ukuze uthole isivuno esiphezulu nekhwalithi. Ngokuqonda ukuthi iyini i-spreader nokuthi sisebenza kanjani, ochwepheshe bokuvutshelwa bangenza izinqumo ezinolwazi mayelana nendlela yokuthuthukisa inqubo yokuvutshelwa futhi bathole imiphumela efiselekayo.

Ingabe unentshisekelo yokuthuthukisa inqubo yakho yokuvutshelwa ngezisakazi?

Uma kunjalo, ungangabazi ukuhlola izinketho ezihlukene zesisakazeli ezitholakalayo ukuze ubone ukuthi zingaluzuzisa kanjani uhlelo lwakho lokusebenza oluthile.

Xhumana nochwepheshe wokuvutshelwa bese ukhetha noma i-OEM right sintered sparger namuhla ukuze ufunde kabanzi futhi uqale ukuthuthukisa inqubo yakho yokuvutshelwa!

Izinsiza ezengeziwe:

Kubafundi abanentshisekelo yokufunda kabanzi mayelana nezisakazi kuma-fermenters, izinsiza ezimbalwa ziyatholakala. Ezinye izinsiza ezinconyiwe zifaka:

"Fermentation Microbiology and Biotechnology" ka-EMT El-Mansi, CFL Amaral kanye ne-WWM Souza (2019)
"Izimiso zobunjiniyela be-Bioprocess" ka-Pauline M. Doran (2012)
"Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells" Umhleli Omkhulu u-Victor G. Aunins (2010)